Plant Tissue Culture: Basic and Applied

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Plant tissue culture in one form or another has become one of the most promising branches of plant science. Arising from the totipotency of plant cells, it now occupies a key position in plant breeding, plant propagation and plant biotechnology. Plant Tissue Culture - Basic and Applied brings to the student accessible, up-to-date information on this subject. Basic knowledge of tissue culture methods such as isolation of suitable tissues from the mother plant, maintenance of the tissues under in vitro condition in an undifferentiated or de-differentiated stage, methods of genetic engineering and gene transfer, chromosomal studies and the handling of in vitro micro plants are described in detail in this book. Similarly, application aspects of micro propagation, haploid cell culture, protoplast culture, embryo culture, somatic embryogenesis and artificial seeds are also discussed.

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Timir Baran Jha is currently Professor of Botany and Head of the Postgraduate and Undergraduate Department of Botany at the Presidency College, Kolkata, a position he has held since May 2002. He has served different government colleges in West Bengal and has twenty-five years of teaching experience in different branches of botany including cell biology, genetics and plant tissue culture at postgraduate and undergraduate levels. He has twenty-seven years of research experience in the basic and applied aspects of plant tissue culture and has published many research papers in national and international journals. He has been a member of the International Association of Plant Tissue Culture and Biotechnology since 1978. Biswajit Ghosh obtained his MSc. and PhD degree in Botany from the University of Calcutta. He has five years of teaching experience in a college under the University of Calcutta. He has eighteen years of research experience in the field of plant tissue culture and has published many research papers in national and international journals. Currently his prime interest is in the field of genetic transformation of higher plants