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Lentivirus Gene Engineering Protocols 14.0%OFF

Lentivirus Gene Engineering Protocols

by Pamela J. Stewart, Maurizio Federico and Maurizio Federico

  • ISBN

    :  

    9781588290915

  • Publisher

    :  

    Humana Press

  • Subject

    :  

    Technology: General Issues, Medical Study & Revision Guides & Reference Material

  • Binding

    :  

    Hardcover

  • Pages

    :  

    328

  • Year

    :  

    2003

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7973.0

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6856.0

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  • Description

    Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.

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