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Antibodies: A Laboratory Manual
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ISBN
:
9780879693145
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Publisher
:
Cold Spring Harbor Laboratory Press
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Subject
:
Biology, Life Sciences, Education
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Binding
:
PAPERBACK
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Pages
:
726
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Year
:
1988
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7421.0
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Description
"[Antibodies aims] to put current immunological methodology into the hands of non-specialists. Happily, Harlow and Lane have succeeded admirably and this manual seems certain also to gain a place on the laboratory bench (too useful to remain on the bookshelf) of a wide variety of biologistseven immunologists. The volume opens with four introductory chapters summarizing key features of the immune response; these provide a succinct overview of the field and are very well written. The scope of the rest of the book includes techniques of immunization and bleeding, monoclonal antibodies and hybridomas, protein labelling, immunoassays (mostly those using solid supports), immunoprecipitation of cellular antigens, immunoblotting and immunoaffinity purification. The methods are presented as easy-to-follow '1-2-3 protocols,' and assume relatively little experience. Rationales for procedures are often presented, which helps to remove some of the mystique that has been associated with immunological methodology."
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